P-108 Does DNA Fragmentation.level significantly impact clinical outcomes in patients undertaking Assisted Reproduction with using donor oocytes?

Abstract Study question Does an abnormal Sperm DNA Fragmentation Index significantly impact clinical outcomes in patients undergoing Assisted Reproduction with ICSI using donor oocytes? Summary answer No statistical difference was seen pregnancy or miscarriage rates between high low fragmentation indexes oocyte donation cycles intracytoplasmic sperm injection. What is known already High levels of are proposed to have adverse on reproduction. While subject debate, generally accepted ranges > 30% considered and associated poorer outcomes, <15% normal. Impact the intermediate range if 15-30% less understood. Potential interventions for elevated DFI include ICSI, lifestyle changes, antioxidant therapy more controversially TESE. It thought that quality oocytes potential repair damage- thus use younger donors higher fertility may improve outcome overcome a DFI. design, size, duration A retrospective cohort analysis performed our database FET treatment January 2016 December 2020, 1370 identified Screening identify those couples where pre- as part workup, analysed 9% these cycle (n = 124). Clinical were recorded including positive HCG, rate, rate ongoing at 12/40 gestation, compared groups. Participants/materials, setting, methods Oocyte baseline measurements tertiary academic centre identified. Donor treatments chosen reduce female factor variation results. 49 cases had normal DFI(<15%), 21 group ( 30). 54 (15-30%) excluded from analysis. Pregnancy groups assess Main results role chance Elevated has been assisted reproduction outcomes. Other studies suggested compensate this, healthy be able negate any detrimental sperm. With autologous gamete treatments, large variations expected prognosis due characteristics such age, ovarian reserve gynaecological disorders endometriosis PCOS. This egg AMH exclusion identifiable pelvic abnormality allows some standardisation cohort, therefore allowing direct assessment male outcome. Positive HCG per embryo transfer same Normal 53.1% (26/49) vs 47.65% (10/21) p 0.68 Similarly there no Rates based level 44.9% (22/49) 47.7% 0.83 Miscarriage test again not impacted by DFI: 30.7% (8/26) 33.3 (3/10) 0.72 The study data demonstrates combination good oocytes, do appear raised Limitations, reasons caution We previously demonstrated fertilisation blastulation unaffected limited relatively small sample routinely measured before treatment. Larger multi-centre increase population power would useful confirmation findings. Wider implications findings Our suggests biochemical, rates, also adversely cycles.This could attributed ability damage when combined selection fertilisation. Trial registration number Not applicable